West Ham United Ladies suffered a 1-0 Essex FA County Cup semi-final defeat at Colchester United on Sunday.
The Hammers were edged out at Shrub End Community Sports Centre in Essex by an Emily Clements' goal with 20 minutes of the second half remaining.
Julia Setford's side, who were also beaten on penalties in the final of last season's competition, lost former Colchester forward Hannah Gowland to an ankle injury before half-time.
West Ham were unable to find an equaliser late on and will now concentrate all their efforts on the FA Women's Premier League Southern Division.
The Ladies return to action next Sunday 24 February, when they travel to Lewes for a 2pm kick-off. West Ham sit fifth in the table, eight points behind leaders Reading but with two games in-hand on the Royals. The Hammers have to play the leaders home and away before the season ends in April.
West Ham United Ladies: Wayne, Mellors, Barling, Revell, Sullivan, Little, Morgan, Merritt, Gowland (Lipley-Hinton), Blanchflower, Bowers